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immunofluorescence staining  (Beyotime)


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    Structured Review

    Beyotime immunofluorescence staining
    Immunofluorescence Staining, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunofluorescence+with+dapi/DAPI/pm40553481-190-0-8
    Average 99 stars, based on 33153 article reviews
    immunofluorescence staining - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Multi-omics analysis reveals NSUN2-mediated m5C methylation of DOCK9 regulating vascular stability in brain arteriovenous malformations.
    Article Snippet: The next day, sections were washed and incubated for 2 h at room temperature in the dark with species-specific secondary antibodies: Alexa Fluor 594-conjugated goat anti-mouse IgG (Abcam, #ab150116, red signal for CD31) and Alexa Fluor 488-conjugated goat anti-rabbit IgG (Abcam, #ab150077, green signal for DOCK9 or NSUN2). .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. To detect CD31, DOCK9, and NSUN2 simultaneously, triple immunofluorescence staining was performed using a tyramide signal amplification (TSA)-based protocol with sequential antibody labeling. .. Tissue sections were first incubated with rabbit anti-DOCK9 (Proteintech, #18987-1-AP), followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody and developed with iF647-Tyramide (Servicebio, G1232), producing a far-red fluorescent signal for DOCK9.

    Article Title: Panaxytriol up-regulates CYP2B6 expression through PXR and CAR and their interactions with RXRα and SRC-1.
    Article Snippet: 2 3 Ethnopharmacological relevance: Cytochrome P450 2B6 (CYP2B6) is a critical drug2 4 metabolizing enzyme, and its expression is regulated by the nuclear receptors.. Panaxytriol 2 5 (PXT), a prominent bioactive constituent extracted from the roots of Panax ginseng C.A.. Meyer, 2 6 was found to induce cytochrome P450 3A4 (CYP3A4) via pregnane X receptor (PXR) and 2 7 constitutive androstane receptor (CAR) in our previous work.

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation
    Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole (Beyotime) for 5 min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Staining:

    Article Title: Multi-omics analysis reveals NSUN2-mediated m5C methylation of DOCK9 regulating vascular stability in brain arteriovenous malformations.
    Article Snippet: The next day, sections were washed and incubated for 2 h at room temperature in the dark with species-specific secondary antibodies: Alexa Fluor 594-conjugated goat anti-mouse IgG (Abcam, #ab150116, red signal for CD31) and Alexa Fluor 488-conjugated goat anti-rabbit IgG (Abcam, #ab150077, green signal for DOCK9 or NSUN2). .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. To detect CD31, DOCK9, and NSUN2 simultaneously, triple immunofluorescence staining was performed using a tyramide signal amplification (TSA)-based protocol with sequential antibody labeling. .. Tissue sections were first incubated with rabbit anti-DOCK9 (Proteintech, #18987-1-AP), followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody and developed with iF647-Tyramide (Servicebio, G1232), producing a far-red fluorescent signal for DOCK9.

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation
    Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole (Beyotime) for 5 min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: CNIH4 regulates systemic metabolism and exercise adaptations in male mice primarily through the modulation of β-adrenergic receptor trafficking
    Article Snippet: After incubation, sections were washed three times with PBST, followed by incubation with goat anti-rabbit Cy3-conjugated secondary antibody (1:500, Thermo, A10520) and goat anti-mouse 488-conjugated secondary antibody (1:500, Thermo, A-11001) for 1 h at room temperature in the dark. .. Coverslips were mounted using Mounting Medium with DAPI (Beyotime, P0131, China) to stain the nuclei. .. Fluorescent images were acquired using an Olympus FV3000 confocal microscope (Japan) equipped with a 20 x objective, with representative images taken from two independent experimental replicates using the cellSens FV Desktop system.

    Amplification:

    Article Title: Multi-omics analysis reveals NSUN2-mediated m5C methylation of DOCK9 regulating vascular stability in brain arteriovenous malformations.
    Article Snippet: The next day, sections were washed and incubated for 2 h at room temperature in the dark with species-specific secondary antibodies: Alexa Fluor 594-conjugated goat anti-mouse IgG (Abcam, #ab150116, red signal for CD31) and Alexa Fluor 488-conjugated goat anti-rabbit IgG (Abcam, #ab150077, green signal for DOCK9 or NSUN2). .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. To detect CD31, DOCK9, and NSUN2 simultaneously, triple immunofluorescence staining was performed using a tyramide signal amplification (TSA)-based protocol with sequential antibody labeling. .. Tissue sections were first incubated with rabbit anti-DOCK9 (Proteintech, #18987-1-AP), followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody and developed with iF647-Tyramide (Servicebio, G1232), producing a far-red fluorescent signal for DOCK9.

    Antibody Labeling:

    Article Title: Multi-omics analysis reveals NSUN2-mediated m5C methylation of DOCK9 regulating vascular stability in brain arteriovenous malformations.
    Article Snippet: The next day, sections were washed and incubated for 2 h at room temperature in the dark with species-specific secondary antibodies: Alexa Fluor 594-conjugated goat anti-mouse IgG (Abcam, #ab150116, red signal for CD31) and Alexa Fluor 488-conjugated goat anti-rabbit IgG (Abcam, #ab150077, green signal for DOCK9 or NSUN2). .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. To detect CD31, DOCK9, and NSUN2 simultaneously, triple immunofluorescence staining was performed using a tyramide signal amplification (TSA)-based protocol with sequential antibody labeling. .. Tissue sections were first incubated with rabbit anti-DOCK9 (Proteintech, #18987-1-AP), followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody and developed with iF647-Tyramide (Servicebio, G1232), producing a far-red fluorescent signal for DOCK9.

    Confocal:

    Article Title: Panaxytriol up-regulates CYP2B6 expression through PXR and CAR and their interactions with RXRα and SRC-1.
    Article Snippet: 2 3 Ethnopharmacological relevance: Cytochrome P450 2B6 (CYP2B6) is a critical drug2 4 metabolizing enzyme, and its expression is regulated by the nuclear receptors.. Panaxytriol 2 5 (PXT), a prominent bioactive constituent extracted from the roots of Panax ginseng C.A.. Meyer, 2 6 was found to induce cytochrome P450 3A4 (CYP3A4) via pregnane X receptor (PXR) and 2 7 constitutive androstane receptor (CAR) in our previous work.

    Laser-Scanning Microscopy:

    Article Title: Panaxytriol up-regulates CYP2B6 expression through PXR and CAR and their interactions with RXRα and SRC-1.
    Article Snippet: 2 3 Ethnopharmacological relevance: Cytochrome P450 2B6 (CYP2B6) is a critical drug2 4 metabolizing enzyme, and its expression is regulated by the nuclear receptors.. Panaxytriol 2 5 (PXT), a prominent bioactive constituent extracted from the roots of Panax ginseng C.A.. Meyer, 2 6 was found to induce cytochrome P450 3A4 (CYP3A4) via pregnane X receptor (PXR) and 2 7 constitutive androstane receptor (CAR) in our previous work.

    Bicinchoninic Acid Protein Assay:

    Article Title: Microglial serine racemase knockout alleviates Alzheimer-like neuropathology and behavioral deficit via lactylation-mediated anti-inflammation
    Article Snippet: .. Penicillin–streptomycin (cat#C0222), amyloid β-peptide (1–42) (cat#P9001), lipopolysaccharide (cat#ST1470), DL-dithiothreitol (cat#ST041), the enhanced BCA protein assay kit (cat#P0010), enhanced BCA protein assay kit (cat #P0010), normal goat serum (cat #C0265), and antifade mounting medium with DAPI (cat #P0131) were purchased from Beyotime Biotechnology, Inc (Shanghai, China). .. Mouse anti-Aβ antibody (6E10; RRID: AB_2564652) was purchased from BioLegend (San Diego, CA, USA).

    Labeling:

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation
    Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole (Beyotime) for 5 min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Blocking Assay:

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation
    Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole (Beyotime) for 5 min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Incubation:

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Article Title: All‐Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27–Mediated Autophagy Modulation
    Article Snippet: .. The cells were fixed in cold 4% paraformaldehyde at room temperature for 10 min. For immunofluorescence labeling, cells were blocked for 10 min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole (Beyotime) for 5 min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).



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